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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
23/12/2014 |
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Data da última atualização: |
23/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CREVELIN, E. J.; CROTTI, A. E. M.; ZUCCHI, T. D.; MELO, I. S. de; MORAES, L. A. B. |
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Afiliação: |
EDUARDO JOSE CREVELIN, FFCLRP-USP; ANTONIO EDUARDO MILLER CROTTI, FFCLRP-USP; TIAGO DOMINGUES ZUCCHI, FAPESP; ITAMAR SOARES DE MELO, CNPMA; LUIZ ALBERTO BERALDO MORAES, FFCLRP-USP. |
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Título: |
Dereplication of Streptomyces sp. AMC 23 polyether ionophore antibiotics by accurate-mass electrospray tandem mass spectrometry. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Journal of Mass Spectrometry, Chichester, v. 49, n. 11, p. 1117-1126, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstracts: Actinomycetes, especially those belonging to the genus Streptomyces, are economically important from a biotechnological standpoint: they produce antibiotics, anticancer compounds and a variety of bioactive substances that are potentially applicable in the agrochemical and pharmaceutical industries. This paper combined accurate-mass electrospray tandem mass spectrometry in the full scan and product ion scan modes with compounds library data to identify the major compounds in the crude extract produced by Streptomyces sp. AMC 23; it also investigated how sodiated nonactin ([M + Na]+) fragmented. Most product ions resulted from elimination of 184 mass units due to consecutive McLafferty-type rearrangements. The data allowed identification of four macrotetrolides homologous to nonactin (monactin, isodinactin, isotrinactin/trinactin and tetranactin) as well as three related linear dimer compounds (nonactyl nonactoate, nonactyl homononactoate and homononactyl homononactoate). The major product ions of the sodiated molecules of these compounds also originated from elimination of 184 and 198 mass units. UPLC-MS/MS in the neutral loss scan mode helped to identify these compounds on the basis of the elimination of 184 and 198 mass units. This method aided monitoring of the relative production of these compounds for 32 days and revealed that the biosynthetic process began with increased production of linear dimers as compared with macrotetrolides. These data could facilitate dereplication and identification of these compounds in other microbial crude extracts. MenosAbstracts: Actinomycetes, especially those belonging to the genus Streptomyces, are economically important from a biotechnological standpoint: they produce antibiotics, anticancer compounds and a variety of bioactive substances that are potentially applicable in the agrochemical and pharmaceutical industries. This paper combined accurate-mass electrospray tandem mass spectrometry in the full scan and product ion scan modes with compounds library data to identify the major compounds in the crude extract produced by Streptomyces sp. AMC 23; it also investigated how sodiated nonactin ([M + Na]+) fragmented. Most product ions resulted from elimination of 184 mass units due to consecutive McLafferty-type rearrangements. The data allowed identification of four macrotetrolides homologous to nonactin (monactin, isodinactin, isotrinactin/trinactin and tetranactin) as well as three related linear dimer compounds (nonactyl nonactoate, nonactyl homononactoate and homononactyl homononactoate). The major product ions of the sodiated molecules of these compounds also originated from elimination of 184 and 198 mass units. UPLC-MS/MS in the neutral loss scan mode helped to identify these compounds on the basis of the elimination of 184 and 198 mass units. This method aided monitoring of the relative production of these compounds for 32 days and revealed that the biosynthetic process began with increased production of linear dimers as compared with macrotetrolides. These data could facilitate... Mostrar Tudo |
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Palavras-Chave: |
Actinomycetes; Macrotetrolides; Nonactic acid; UPLC-MS/MS. |
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Thesagro: |
Actinomiceto; Espectrometria. |
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Thesaurus Nal: |
Actinomycetales; DNA fragmentation; Extracts; Streptomyces; Tandem mass spectrometry; Ultra-performance liquid chromatography. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114323/1/2014AP035.pdf
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Marc: |
LEADER 02561naa a2200313 a 4500
001 2003573
005 2014-12-23
008 2014 bl uuuu u00u1 u #d
100 1 $aCREVELIN, E. J.
245 $aDereplication of Streptomyces sp. AMC 23 polyether ionophore antibiotics by accurate-mass electrospray tandem mass spectrometry.$h[electronic resource]
260 $c2014
520 $aAbstracts: Actinomycetes, especially those belonging to the genus Streptomyces, are economically important from a biotechnological standpoint: they produce antibiotics, anticancer compounds and a variety of bioactive substances that are potentially applicable in the agrochemical and pharmaceutical industries. This paper combined accurate-mass electrospray tandem mass spectrometry in the full scan and product ion scan modes with compounds library data to identify the major compounds in the crude extract produced by Streptomyces sp. AMC 23; it also investigated how sodiated nonactin ([M + Na]+) fragmented. Most product ions resulted from elimination of 184 mass units due to consecutive McLafferty-type rearrangements. The data allowed identification of four macrotetrolides homologous to nonactin (monactin, isodinactin, isotrinactin/trinactin and tetranactin) as well as three related linear dimer compounds (nonactyl nonactoate, nonactyl homononactoate and homononactyl homononactoate). The major product ions of the sodiated molecules of these compounds also originated from elimination of 184 and 198 mass units. UPLC-MS/MS in the neutral loss scan mode helped to identify these compounds on the basis of the elimination of 184 and 198 mass units. This method aided monitoring of the relative production of these compounds for 32 days and revealed that the biosynthetic process began with increased production of linear dimers as compared with macrotetrolides. These data could facilitate dereplication and identification of these compounds in other microbial crude extracts.
650 $aActinomycetales
650 $aDNA fragmentation
650 $aExtracts
650 $aStreptomyces
650 $aTandem mass spectrometry
650 $aUltra-performance liquid chromatography
650 $aActinomiceto
650 $aEspectrometria
653 $aActinomycetes
653 $aMacrotetrolides
653 $aNonactic acid
653 $aUPLC-MS/MS
700 1 $aCROTTI, A. E. M.
700 1 $aZUCCHI, T. D.
700 1 $aMELO, I. S. de
700 1 $aMORAES, L. A. B.
773 $tJournal of Mass Spectrometry, Chichester$gv. 49, n. 11, p. 1117-1126, 2014.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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| Registros recuperados : 1 | |
| 1. |  | SILVA, D. C. da; ALVES, A. A.; VASCONCELOS, V. R.; NASCIMENTO, H. T. S. do; MOREIRA FILHO, M. A.; OLIVEIRA, M. E. de. Metabolismo dos compostos nitrogenados em ovinos alimentados com dietas contendo farelo de mamona destoxificado. Acta Scientiarum. Animal Sciences, Maringá, v. 32, n. 2, p. 219-224, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 1 | |
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Registro completo
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Biblioteca(s): |
Catálogo Coletivo de Periódicos Embrapa; Embrapa Amazônia Oriental; Embrapa Gado de Corte. |
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Identificador: |
855 |
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Data corrente: |
09/05/2002 |
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Data da última atualização: |
20/05/2015 |
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Código do título: |
1800010 |
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ISSN: |
0307-8035 |
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Código CCN: |
064290-8 |
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Título e Subtítulo: |
ANNUAL REPORT OF STUDIES IN ANIMAL NUTRITION AND ALLIED SCIENCES |
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Título anterior: |
ANNUAL REPORT ON ANIMAL NUTRITION AND ALLIED SCIENCE |
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Entidade: |
Reid Library |
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Local de publicação: |
Aberdeen, Escocia, GB |
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Periodicidade: |
Anual |
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Inicio de publicação: |
1966 |
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Coleções da unidade: |
Embrapa Amazônia Oriental
1970 26; 1971 27; 1972 28; 1973 29; 1974 30; 1975 31; 1976 32; 1977 33; 1978 34; 1979 35; 1980 36; 1981 37; 1982 38; 1983 39; 1985 40; 1986 41
Classificação: 636.0852005A613
Embrapa Gado de Corte
1977-83 33-39;
Classificação: 636.005 |
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